分光光度法测定微乳液中脂肪酶的酶活

Spectrophotometric Assay of Lipase Activity in Microemulsion

通过对胶束酶学对传统脂肪酶酶活测定的改进 ,建立了适合于油包水微乳液体系中脂肪酶酶活测定的分光光度方法。与传统方法相比 ,本法克服了底物乳化液难配制、不稳定等缺点 ,无需进行在pH缓冲溶液体系中的酸碱滴定 ,提高了酶活测定的准确性。

Based on micellar enzymology,a modified experiment on lipase assay is described in this report,which is intended for these students who major or minor in experimental biochemistry.The present method has some merits over the conventional one in the medium and technique used.\;