转化生长因子-β_1对前列腺基质细胞基因表达的调节作用

TGF-β_1 regulates the expression of multiple genes in prostatic stromal cells in vitro

目的 探讨转化生长因子 β1(TGF β1)对前列腺基质细胞基因表达的调节作用。 方法 原代培养人前列腺基质细胞 ,并传代至 4～ 6代。分别将浓度为 0 .0 1、0 .10、1.0 0、10 .0 0 μg/L的TGF β1加入细胞培养液中。孵育 48h后收集细胞。用内参照半定量RT PCR方法检测前列腺基质细胞雄激素受体 (AR)、TGF β1、bFGF和平滑肌特异性标记蛋白smoothelin基因的转录水平。 结果 与对照组相比 ,低浓度 (0 .0 1μg/L)的TGF β1可增强体外培养的前列腺基质细胞内AR表达 (P <0 .0 1) ,差别有显著性意义。随TGF β1浓度增加 ,促AR表达作用减弱。随TGF β1浓度增加基质细胞TGF β1、bFGF和smoothelin基因的转录增加 (P <0 .0 1) ,并且随着应用浓度增加促各基因转录的作用增强。 结论 TGF β1对前列腺基质细胞基因表达具有广泛的调节作用 。

Objective To study the regulatory effect of TGF β 1 on the expression of multiple genes in prostatic stromal cells in vitro. Methods The primary culture of prostatic stromal cells (including fibroblasts and smooth muscle cells ) have been established and cultured to 4～6 passages. Then the cells were cultured in the medium with various concentration of TGF β 1(0.01, 0.10, 1.00 and 10.0 ng/ml)for 48h. By semi quantitative RT PCR method, the androgen receptor(AR), TGF β 1, bFGF and smoothlin mRNA were measured. Results The expression of AR could be stimulated by low concentration of TGF β 1 ( P <0.01), whereas the stimulated effect gradually dropped with increasing TGF β 1 concentration. TGF β 1 could also enhance the expression of TGF β 1, bFGF and smoothlin gene ( P <0.01) in a dose related pattern. Conclusions TGF β 1 was a modulating factor for the expression of multiple genes of prostatic stromal cells. This may explain the role of TGF β 1 in the pathogenesis of BPH.