异常瘢痕成纤维细胞在低血清及白介素1_β作用下的细胞凋亡研究

Study on apoptosis of fiberblasts from abnormal scars in media containing low level of serum and interleukin 1β

目的 明确低血清及白介素 1β(IL - 1β)对瘢痕疙瘩、增生性瘢痕及正常皮肤成纤维细胞诱导细胞凋亡的作用。方法 对 6例瘢痕疙瘩、6例增生性瘢痕及 6例正常皮肤标本采用细胞培养、免疫组织化学及凝胶电泳方法 ,通过检测Bax、Bcl 2蛋白及特异性DNA梯形条带 ,对不同成纤维细胞在低血清中及IL 1β作用后的细胞凋亡进行了研究。结果 ①在低血清中 ,瘢痕疙瘩和增生性瘢痕成纤维细胞的Bax Bcl 2蛋白表达比值没有明显改变 ,但增生性瘢痕成纤维细胞出现程度较轻的细胞凋亡 ,而瘢痕疙瘩成纤维细胞未见明显细胞凋亡 ;正常皮肤成纤维细胞出现细胞凋亡 ,且Bax Bcl 2比值升高 ,表明发生细胞凋亡。②IL 1β作用下 ,三者成纤维细胞均发生凋亡 ,瘢痕疙瘩和增生性瘢痕凋亡比正常皮肤严重 ,但Bax Bcl 2比值在瘢痕疙瘩升高 ,在正常皮肤降低 ,增生性瘢痕无明显变化。结论 不同的成纤维细胞特性存在差异。

Objective To investigate the effects of low serum and interleukin 1β on inducing apoptosis of fibroblasts from abnormal scars and normal skins. Methods\ 6 samples from keloid, hypertrophic scar and normal skin were collected respectively. Apoptosis of different fibroblasts in media containing low level of serum and interleukin 1β were studied by detecting Bax and Bcl\|2 protein expressions and specific DNA ladder by means of cell culture, immunohistochemistry and agrose electrophoresis. Results\ ①In low level of serum, hardly occurs apoptosis of fibroblasts from keloids in correspondence with no changes of the radio of Bax/Bcl 2 proteins, and apoptosis of fibroblasts from normal skins occurs in correspondence with increasing of the radio of Bax/Bcl 2 proteins, whereas, slightly occurs apoptosis of fibroblasts from hypertrophic scars in correspondence with no change of the radio of Bax/Bcl 2 proteins. ②In media containing interleukin 1β apoptosis of all three kinds of fibroblasts occurs, whereas, apoptosis of fibroblasts from keloids and hypertrophic scars takes place more seriously than that from normal skins in correspondence with increasing of the Bax/Bcl 2 radio of fibroblasts from keloids, decreasing of the Bax/Bcl 2 radio of ones from normal skins and no change of the Bax/Bcl 2 radio of ones from hypertrophic scars. Conclusion\ Characters of different fibroblasts are different.\;