树突状细胞在异常瘢痕中的免疫调控作用

Immunological regulations of dendritic cell in abnormal scarring tissue

目的 探讨树突状细胞与异常瘢痕发病机理的关系。方法 采用免疫组织化学方法检测 6例增生性瘢痕 (HS)、6例瘢痕疙瘩 (K)和 6例正常皮肤DC表面分子HLA DR和CD1a的含量 ,并观察激素对HSHLA DR和CD1a 的影响。结果 ①HS和K组织中表皮HLA DR+ DC的数量( 80 6 67± 10 1 72 )个 mm2 和 ( 870 0 0± 13 4 2 4 )个 mm2 ,明显高于正常皮肤 (P <0 0 5 ) ,HLA DR分子在角质形成细胞和成纤维细胞中异常表达。②HS和K组织中表皮CD1a+ DC的数量 ( 70 0 0 0± 97 2 3 )个 mm2 和 ( 780 0 0± 10 4 4 7)个 mm2 ,明显高于正常皮肤 (P <0 0 5 )。③激素治疗 3 ,7d时 ,HS表皮HLA DR+ DC的数量 ( 4 76 67± 70 0 2 )个 mm2 和 ( 4 47 76± 90 0 3 )个 mm2 ,CD1a+ DC的数量 ( 4 5 6 3 6±82 88)个 mm2 和 ( 3 96 18± 99 3 6)个 mm2 ,明显降低 (P <0 0 5 )。结论 ①HLA DR和CD1a分子表达量增加 ,提示HS和K局部组织可能处于高免疫应答状态 ;②激素可能通过抑制HLA DR和CD1a分子的表达量降低HS高免疫应答状态。

Objective To investigate the relationship between dendritic cell(DC) and pathogenesis of abnormal scar. Methods\ The content of HLA DR and CD 1a molecules of DC, in 6 samples of hypertrophic scar(HS), keloid (K) and normal skin, were determined with the stain of avidin biotin peroxidase complex method(ABC). The effect of Triamcinolone Acetonide was evaluated with the measurement of the HLA DR and CD 1a molecules of DC in the epidermis of the HS. Results\ ①The amounts of HLA DR molecules of the positive DC were 806.67±101.72 and 870.00±134.24 in the HS and the K respectively, significantly higher than the controlled normal skin (510.01±45.17, P <0.05). HLA DR molecules showed an abnormal expression in the Kerationcytes and fibroblasts. ②The amounts of CD 1a molecules of the positive DC were 700.00±97.23 and 780.00±104.47 in the HS and the K respectively, significantly higher than the controlled normal skin (521.24±57.87) ( P <0.05). ③The amounts of the HLA DR molecules positive DC, in the positive kerationcytes and fibroblasts of hypertrophic scar, treated by Triamcinolone Acetonide, were 476.67±70.02 and 447.76±90.03 ( P <0.05) for 3 days and 7 days treatment respectively, significantly lower than the control. The amounts of CD 1a molecules positive DC in the epidermis of hypertrophic scar with the injection of Triamcinolone Acetonide were significantly lower in 3 days and 7 days treatment (456.36±82.88 and 397.18±99.36, P <0.05). Conclusion\ ①The results, with the high expression of HLA DR and CD 1a molecules, indicate that the HS and the K may have strong immune reactions. ②Triamcinolone Acetonide may decrease the immune reactions of the HS, through the inhibition of the expressions of HLA DR and CD 1a molecules in the dendritric cell.;