慢性乙型病毒性肝炎患者外周血细胞CCR5及CD30的表达

CCR5 and CD30 expression of peripheral cell in patients with hepatitis B

目的 为研究细胞趋化因子受体 5 (CCR5 )及细胞分化抗原 30 (CD30 )作为 1类辅助性T细胞 /细胞毒性T细胞 (Th1/Tc1)、2类辅助性T细胞 /细胞毒性T细胞 (Th2 /Tc2 )表面标志的可行性及辅助性T细胞 (Th) /细胞毒性T细胞亚群 (Tc)在病毒性肝炎免疫发病机制中的作用。方法对 2 0例正常人群 (NC)及 2 0例慢性乙型病毒性肝炎 (CHB)患者外周血单核细胞 (PMBC)经植物血凝素 (PHA)培养前后CCR5、CD30的表达水平进行了检测。结果 ①培养前 ,NC与CHB患者PM BC的CCR5及CD30的表达无明显的差别 (P >0 0 5 ) ,但两组CCR5的表达均高于CD30 (P <0 0 5 )。②培养后 ,CHB患者CCR5高于NC(P >0 0 5 ) ,而CD30则低于NC(P <0 0 5 )。③培养后 ,NC的CCR5表达较培养前明显的减少 (P <0 0 5 ) ,而NC及CHB患者的CD30的表达较培养前均明显的增加 (P <0 0 5 ) ;培养后 ,CHB患者CCR5的减少量低于NC ,而CD30的增加量少于NC(P <0 0 5 )。④培养前CHB患者CCR5 /CD30低于NC ,而培养后高于NC(P <0 0 5 ) ,两组培养后CCR5 /CD30均明显低于培养前 (P <0 0 5 )。结论 ①CCR5及CD30目前可作为Th及Tc细胞亚群相对特异性的细胞表面标志物 ;

Objective To study that if CCR5 and CD30 could be respectively used as the cell surface markers of 1,2 type of T helper and cytotoxic cell (Th1/Tc1,Th2/Tc2)and the roles of Th/Tc subtype cells in the immunological pathogenesis of viral hepatitis.Methods Pre-PHA-cultivated and post-PHA-cultivated expression levels of CCR5 and CD30 of PMBC of 20 hepatitis B patients and 20 normal subjects were assayed by flow cytometry.Results Before cultivation,there was no discrepancy between NC and CHB,but the CCR5 levels of the two groups were higher than that of CD30;After cultivation,CCR5 of CHB was higher than that of NC,but CD30 was lower than NC;CD30,CDR5 and CCR5/CD30 levels between pre-cultivation and post-cultivation had obvious discrepancy.Conclusion ①CCR5 and CD30 could be used as the specific markers of Th,Tc subtypes.②Abnormal or disorder expression levels between CCR5 and CD30 might have some relationship with the pathogenesis of viral hepatitis.