龟板对局灶性脑缺血后神经干细胞的作用

Effect of Plastrum Testudinis on Neural Stem Cell After Focal Cerebral Ischemia

【目的】探讨补肾中药龟板对局灶性脑缺血后神经干细胞的作用。【方法】采用大脑中动脉线栓法造成局灶性脑缺血大鼠模型 ,应用免疫组织化学技术检测神经上皮干细胞蛋白 (Nestin)的表达 ,观察龟板对脑缺血后神经上皮干细胞蛋白的影响。【结果】龟板可降低神经病评分和增强脑缺血后Nestin表达。【结论】提示补肾中药龟板可减轻神经损伤症状和对局灶性脑缺血后神经干细胞有促进增殖作用。

Objective To investigate the effect of Plastrum Testudinis on neural stem cell after focal cerebral ischemia.Methods Rat models of focal cerebral ischemia were established by inserting nylon thread into the anterior cerebral artery.Immunohistochemical method was used to detect the expression of the neuroepithelial stem cell protein(Nestin),neurologic deficit and histological features were also observed.Results Plastrum Testudinis can decrease the neurologic dificit score and promote the expression of Nestin.Conclusion Plastrum Testudinis can alleviate the neurological symptoms and promote the proliferation of neural stem cell after focal cerebral ischemia.