黄芪注射液对实验性大鼠腹膜透析并发腹膜炎的影响

Effect of Astragali Injection on Rats with Peritoneal Dialysis Complicated with Peritonitis

【目的】在普通腹透液中加入黄芪注射液 ,进行腹膜透析 ,观察其对透析并发腹膜炎的疗效及对腹腔防御机制的影响。【方法】按随机法将 4 0只清洁级SD大鼠分为黄芪透析模型组、常规透析模型组、黄芪透析正常组、常规透析正常组 4组 ,每组 10只 ,以腹腔注入大肠杆菌的方法造成腹膜炎模型 ,分别以含生药8g L黄芪注射液的腹透液和不含黄芪注射液的普通腹透液对模型和正常大鼠透析 ,连续 7d ,并观察各组动物的一般情况 ,包括精神状态、进食、大小便 ,以及有无寒战、烦躁等临床症状 ,透析结束后检测腹透液常规、腹透液细菌培养 +菌落计数及大鼠腹腔巨噬细胞功能。【结果】黄芪透析模型组、黄芪透析正常组及常规透析正常组腹透液白细胞总数、中性粒细胞数均显著低于常规透析模型组 (P<0 .0 5或 P<0 .0 1) ,而前 3组间差异无显著性 ;各组腹透液蛋白质浓度无明显差异 (均 P>0 .0 5 ) ;常规透析模型组的巨噬细胞吞噬百分率及吞噬指数显著低于黄芪透析模型组、黄芪透析正常组及常规透析正常组 (P<0 .0 5或 P<0 .0 1) ;黄芪透析正常组的吞噬百分率显著高于常规透析正常组 ,而两组的吞噬指数则无显著性差异。【结论】含黄芪注射液的腹透液透析确能减轻腹膜透析腹膜炎的发病程度 ,其机理可能通过免疫调节途径 。

Objective To observe the effect of routine dialysate (RD) added with Astragali Injection (AI) on rats with peritoneal dialysis complicated with peritonitis and to study its defense mechansim.Methods Forty clean SD rats were allocated to four groups: RD added with AI model group (Group A), RD in model group (Group B), AI in normal group (Group C)and RD in normal group (Group D). Rat model with peritonitis was established by intraperitoneal injection of Bacillus coli. The treatment course lasted 7 days. The mental state, appetite, urine and stool, and symptoms such as chill and restlessness were observed. After dialysis, routine examination and bacterial culture of peritoneal dialysate were carried out. The function of macrophyge in abdominal cavity was also detected.Results The number of white blood cell and neutrophil in dialysate were much lower in Group A, Group C and Group D than those in Group B(P< 0.05? or P< 0.01),while the differences among Group A, Group C and Group D were not significant. No significant difference of protein concentration was found among the four groups. Percentage of phygocytosis and phygocytic index of macrophyge were lower in Group B than those in Group A, Group C and Group D (P< 0.05? or P< 0.01).Percentage of phygocytosis was higher in Group C than that in Group D (P< 0.01), while the difference of phygocytic index between the two groups was not significant.Conclusion RD added with AI can improve peritoneal dialysis complicated with peritonitis, its mechanism may be related the regulation of immune function and the increase of phygocytosis of macrophyge and defense function in abdominal cavity. No obvious stimulation was found in the study.