冠心病患者血小板、红细胞蛋白激酶C表达的研究

Expression of Protein Kinase C in Platelets and Erythrocytes from Patients with Coronary Heart Disease

目的：探讨不稳定性心绞痛（ＵＡＰ）和急性心肌梗死（ＡＭＩ）患者血小板、红细胞蛋白激酶Ｃ（ＰＫＣ）的改变。 方法：共测定９８例患者，其中４７例ＵＡＰ组患者，５１例ＡＭＩ组患者，以及５０例健康对照（ＨＣ）组血小板及红细胞胞膜、胞浆ＰＫＣ、血小板胞浆蛋白激酶Ｃ抑制剂（ＰＫＣＩ）活性。 结果：ＵＡＰ组和ＡＭＩ组血小板胞膜中ＰＫＣ活性高于ＨＣ组，胞浆中ＰＫＣ活性低于ＨＣ组，ＵＡＰ组及ＡＭＩ组红细胞胞膜中ＰＫＣ活性高于ＨＣ组，胞浆中ＰＫＣ活性低于ＨＣ组，ＵＡＰ组及ＡＭＩ组血小板胞浆中ＰＫＣＩ活性低于ＨＣ组，均有极显著性差异帜均＜０．０１）。 结论：冠心病ＵＡＰ和ＡＭＩ患者血小板及红细胞ＰＫＣ活性增高，胞浆内ＰＫＣ向细胞膜转移。

Objective: To study the relation between protein kinase C (PKC)and coronary heart disease. Methods: We measured the activity of PKC and its inhibitor (PKCI)in platelets, the activity of PKC in erythrocytes from 47 patients with unstable angina pectoris (UAP), 51 patients with acute myocardial infarction (AMI), and 50 healthy controls (HC), respectively. Results: The activity of PKC in membrane of platelet from UAP and AMI patients was higher than that of HC patients. The activity of PKC in cytosol of platelet from UAP and AMI patients was lower than that from HC patients. The activity of PKCI in cytosol of platelet from UAP and AMI patients was lower than that from HC patients. The activity of PKC in mem- brane of erythrocytes from UAP and AMI patients was higher than that from HC patients. The activity of PKC in cytosol of erythrocytes from UAP and AMI patients was lower than that from HC patients. Conclusion: PKC is likely to be associated with the pathogenesis of coronary heart disease.