全光喷雾和生长调节剂对杜仲插穗的影响

Effects of Growth Regulator,Full Exposure and Mist Spray on the Cutting of Eucommia ulmoides

目的研究提高杜仲生根率的方法。方法将4年生(4y)和20年生(20y)杜仲母树的插穗用清水、α-萘乙酸、N6苄基腺嘌呤和α-萘乙酸+N6苄基腺嘌呤浸泡4h后分别在普通荫棚下和全光喷雾下培养45～60d。定期采样观察插穗的生长情况,培养结束后进行发根率、发根数量、最长根长、愈伤组织、叶片存留数、死苗率等项目的测定和统计。结果在全光喷雾条件下,与4y插穗相比,20y插穗效果更显著,比其在普通条件下的对照组插穗发根率提高13倍,根的性状(根长、根数)明显好于对照,培养时间缩短15d。杜仲插穗生根主要从茎的皮层直接发出不定根,少量从愈伤组织上生根。生根的插穗愈伤组织都不多,过多则影响不定根发生。全光喷雾和生长调节剂处理的插穗可促进不定根直接生成,也促进愈伤组织脱分化发根。结论实验证明全光喷雾和生长调节剂对不同年龄母树插穗的不定根发生均有明显的促进作用,对愈伤组织发生有一定的诱导作用。

Objective To study the effects of growth regulator on the cutting method of Eucommia ulmoides see dlings.Methods Treated with H 2 O(CK),NAA,6-BA and NAA+6-BA for4hours,respectively,cuttings of4-year and20-year trees were cultured for45～60days under the shed or full exposure and mist spray.The growth of the cuttings was observed periodically.The number of the roots,the root length,the callus and the death rate were investigated after harvest.Results Compared with the cutting from4-year-old tree,the cutting from20-year-old tree developed better.The rate of stroking root in treated group was14times as much as CK group.Other agronomic traits such as the number of the roots,the root length,the callus,the number of the leaves and the death rate of the cuttings in treated group were all better than that of the cuttings in CK group.The cultural time in treated group reduced by15days than that of CK group.Conclusions The present study shows that both full exposure mist spray and growth regulators have remarkable effects on cuttings of tree with different ages for rooting or form ing callus.