期刊文献+

人乳头瘤病毒6型基因组晚期区序列分析 被引量:3

Sequence Analysis of the Late Region of Human Papillomavirus Type 6 Genome
下载PDF
导出
摘要 目的初步分析我国尖锐湿疣患者病变组织分离的乳头瘤病毒6型(HPV-6)基因组晚期区编码序列变异情况。方法采用重叠PCR设计,分别从HPV-6阳性尖锐湿疣患者病变组织扩增出主要衣壳蛋白L1和次要衣壳蛋白L2基因片段,插入质粒载体克隆,经测序、拼接获得HPV-6基因组晚期区序列。结果得到的两条长2869bp的HPV-6晚期区序列(GenBank序列索取号AY015006,AY015008)均具有完整的L1和L2开放阅读框(ORF),与原型序列比较,在L1和L2ORF中共有9个核苷酸变异,其中错义突变4个(有3个发生在L2ORF,1个在L1ORF)。分子进化分析表明,克隆的晚期区序列属于HPV-6b亚型。结论HPV-6基因组晚期区编码序列非常保守(核苷酸变异率小于0.28%),并且L1ORF比L2ORF更为保守,其中位于L1ORF中7081位的A→G和7099位的G→A两处共有突变在我国HPV-6分离物中具有代表性。本研究首次从我国尖锐湿疣组织标本克隆了HPV-6基因组晚期区全长编码序列。 Objective To study variations of genome late region of human papillomavirus type6(HPV-6)isolated from Chinese patients with condyloma acuminatum.Methods Using overlap PCR design,major capsid protein(L1)and minor capsid protein(L2)genes were separately amplified from clinical sa mpl es following HPV type determination,and were further assembled into HPV-6genome late region sequences after inserting into plasmid and sequencing.Results Two sequences(GenBank accession nu-mber AY015006,AY015008)of HPV-6late region were assembled,which are2869bp long covering35%HPV-6genome and with complete open reading frames(ORFs)for L1and L2.Compared with prototype sequence,nine point mutations were found,including four missense mutations,three of which were located in L2ORF.Phylogenetic analysis indicated that the cloned sequences are classified into HPV-6b.Conclusions HPV-6genome late region,especially in L1ORF,is very conserved(variation rate<0.28%).The mutation from A to G at position7081in HPV-6genome and from G to A at7099may represent region characteristics.This is the first report that describes sequence variations among genome late region of HPV-6isolated from Chinese patients.
出处 《中国医学科学院学报》 CAS CSCD 北大核心 2001年第6期568-572,共5页 Acta Academiae Medicinae Sinicae
基金 国家自然科学基金(39780011)资助
关键词 尖锐湿疣 人乳瘤病毒6型 晚期区序列分析 克隆 condyloma acuminatum human papillomavirus type6 late region
  • 引文网络
  • 相关文献

参考文献3

共引文献16

同被引文献13

引证文献3

二级引证文献5

;
使用帮助 返回顶部