太子参(PSEUDOSTELLARIA HETEROPHYLLA)病毒病病原鉴定

STUDIES OF TAIZHISHENG (PSEUDOSTELLARIA HETEROPHYLLA)VIRUS DISEASES

太子参(Pseudostellaria heterophylla)是我国传统的中药材之一,其病毒病十分严重,田间发病率和种根带毒率可达100％,平均90％左右。自太子参病株分离得到的T-17、T-32、T-69和T-01四个病毒分离物,经鉴定,它们分别为芜菁花叶病毒(Turnip mosaicvirus,TuMV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)、蚕豆萎蔫病毒(Broad beanwilt virus,BBWV)和烟草花叶病毒(Tobacco mosaic virus,TMV)。其中以TuMV分布最广,为害最重,是太子参病毒病的主要病原。

Taizhisheng is a Chinese traditional medicinal herb. In recent years, virus diseases have been found on it. The infected plants appeared severely mosaic, crinkled and stunted. Investigations were carried out in 1984-1987 in Jiangsu Province and Sh- andong Province. The diseases were wildely distributed with an infection rate over 90%. Four virus isolates, T-17, T-32, T-69, T-01, were obtained from infected plants. They were identified as Turnip mosa'c virus, Cucumber mosaic virus, Broad bean wilt virus, Tobacco mosaic virus, respectively, according to host range, host reactions and symptoms, virus stability in sap, mode of transmission by vector, practical morphology and size, type of inclusion bodies within infected plant cells and serological test. Am- ong four isolates, the TuMV was the dominant one which was wide distributed and caused severe mosaic and crinkle; the CMV was also wide distributed and caused light mosaic; the BBWV was only isolated in northern Jrangsu Province, the TMV was only found in Shandong Province and Jiangsu Province and induced masking infection or light mosaic.