rhIL-1β及TGF-β对髁状突软骨细胞增殖影响的研究

Effects of RhIL-1β and TGF-β on Proliferation of Condylar Chondrcytes.

目的 :探讨rhIL 1β及TGF β对髁状突软骨细胞增殖的影响。 方法 :以酶消化法获得兔髁状突软骨细胞。用MTT法及PCNA免疫组化染色检测不同浓度rhIL 1β及TGF β对体外培养的兔髁状突软骨细胞增殖的影响。结果 :MTT检测 ,rhIL 1β( 10、2 0ng/ml)处理软骨细胞 4 8h后 ,可明显抑制软骨细胞增殖 ,经统计学检验 ,处理 3～ 6d有显著性差异 (P <0 .0 1,t检验 ) ;加入TGF β( 10、2 0ng/ml)可明显拮抗rhIL 1β对软骨细胞增殖的抑制作用 ,并且浓度愈高对rhIL 1β的抑制作用愈强。PCNA检测 ,rhIL 1β处理软骨细胞后PCNA阳性细胞率降低 ,随rhIL 1β的刺激浓度增加 ,阳性率明显降低 ;加入外源性TGF β可以使PCNA的阳性率提高。 结论 :rhIL 1β对髁状突软骨细胞增殖具有抑制作用 ,外源性TGF

To investigate effects of recombinant human interleukin 1β(rhIL 1β)and exgenous transforming growth factor β(TGF β)on proliferation of condylar chondrcytes in vitro.Methods:The proliferation of condylar chondrocytes were examined with MTT assay and immunohistochemical staining of proliferating cell nuclear antigen(PCNA)when different concentration rhIL 1β and TGF β was supplemented in culture.Results:The significant suppressive role of proliferation was observed on condylar chondrcytes after treated with rhIL 1β(10ng?20ng/ml) 48h,proliferation was enhanced by TGF β(10ng?20ng/ml).The positive index of PCNA was decreased or increased by rhIL 1β or TGF β.Conclusions:rhIL 1β suppressed proliferation of condylar chondrocytes,TGF β could antagonized the role of rhIL 1β.