人内皮生长抑制素基因的克隆研究

Study on Cloning Human Endostatin Gene

从人胎肝组织中提取总mRNA ,设计 2对特异引物 ,通过RT PCR获得了人源内抑素基因 ,将其分别插入克隆载体 pBluescript和表达载体pGEX 4T 1中以构建重组质粒。

In order to obtain the human endostatin gene, the total RNA was isolated from human fetus liver tissue, and two pairs of specific primers were designed for RT PCR. The target DNA fragment was inserted into the clone vector pBluescript and the expression vector pGEX 4T 1 respectively. The recombinant plasmids were used for sequencing.The result showed that the DNA sequence of inserted fragment was consistent with human endostatin mRNA in GenBank database.