合成肽RGD和YIGSR的聚合衍生物对PG细胞侵袭重组基底膜能力的影响

The invasive capability of human lung great cellular xancerous PG cell on reformed basement membrane and Inhibition of synthetic peptides

目的 探讨 ＲＧＤ和 ＹＩＧＳＲ的聚合衍生物对 ＰＧ细胞侵袭重组基底膜能力的影响。方法 利用 Ｔｒａｎｓｗｅｌｌ细胞培养小室。结果 适量 ＰＧ细胞经趋化剂作用 １０ｈ，侵袭细胞已达便于统计分析的数量范围；２５～１００μｇ／ｍｌ的合成肽 ＲＧＤ、ＹＩＧＳＲ及多数衍生物与细胞作用 ４８ｈ可对抗 ＰＧ细胞对重组基底膜的侵袭。结论 高转移性人肺白细胞癌 ＰＧ细胞系是肿瘤细胞体外侵袭实验筛选有关抗肿瘤转移药物的适用细胞。其中 ＲＧＤ和 ＹＩＧＳＲ的杂叉聚合肽及 ＹＩＧＳＲ均叉聚合肽的抗肿瘤侵袭活性较强。

Objective To study the invasive capability of human lung great cellular carcinoma PG cell on reformed basement membrane in vitro and inhibition of anti-tumor synthetic peptide. Methods Use tran- swell culture cell. Results When optimun PG cell was affected for lob by chemotatic reagent, invasive cell could reach the number range of statistics; 25-100 ug/ml RGD-YIGSR and most of the derivative could inhibit the invasion of PG cell on reformed basement membrane incubating with cell for 48h. Equal/une- qual-forkpeptide of YLGSR with RGD had much activities of anti-tumor invasion. Conclusion High transfer- able human lung leucocyte PG celluar sery was the usage one of cancerous cell external invasive experimen- tal scalp about anti-cancer tranferable medicine.