外源性p53基因增加卵巢癌细胞对顺铂的敏感性

Enhanced sensitivity of human ovarian cancer cells to cisplatin by exogenous p53 gene

目的 探讨外源性ｐ５３基因转染对人卵巢癌细胞株的化疗敏感性的影响。方法用脂质体介导的转染技术，将人野生型ｐ５３基因的真核表达载体导入不表达ｐ５３的卵巢癌ＳＫＯＶ－３细胞中，经Ｇ４１８筛选，Ｎｏｒｔｈｅｒｎｂｌｏｔ及Ｗｅｓｔｅｒｎｂｌｏｔ鉴定后，观察其对顺铂作用后的 ＳＫＯＶ－３细胞的集落形成及凋亡的影响。结果 外源性Ｐ５３基因在转染细胞中有效表达，并增强了顺铂对ＳＫＯＶ－３细胞集落形成的抑制作用及促进了顺铂诱导的细胞凋亡。结论 外源性ｐ５３基因能增加卵巢癌细胞对顺铂的敏感性，两者联合作用能更大程度地杀灭肿瘤细胞。

Objective To explore the effects of exogenous p53 gene on chemosensitivity of human ovarian cancer cell line SKOV-3. Methods human wild-type p53 gene eukaryotic expression vector was introduced into SKOV-3 cells by lipofectin-mediated gene transfection and the expression of p53 gene in them was de- tected by Northern blot and Western blot. The effects of cisplatin to the transfected cells was observed by colony formation and apoptosis. Results The exogenous p53 gene was expressed effectively in the cells. The expression on p53 gene enhanced the suppression of cisplatin on colony formation and the apoptosis intro- duced by it. Conclusion Exogenous wild-type p53 gene can enhance the sensitivety of human ovarian cancer cells to cisplatin. The combination of p53 gene therapy with chemotherapy may be more effective in cancer treatment.