算盘子对溃疡性结肠炎大鼠细胞因子的影响

Effects of Glochidion Puberum on the Production of TNFα and IL-6 in Macrophage from Mice with Ulcerative Colitis

目的 :探讨中药算盘子提取物对溃疡性结肠炎 (UC)大鼠模型细胞因子的影响。方法 :用 5 %乙酸经结肠灌注制备UC大鼠模型 ,分成对照组及药物组各 5只 ,选择未出现血性腹泻的大鼠 5只作为正常组 ,正常组和对照组每天灌服 0 .9%NaCl溶液 2次 ,每次 10mL ,药物组每天灌服算盘子样品液 2次 ,每次 10mL ,3周后处死 ,按ELISA法测定巨噬细胞中肿瘤坏死因子 (TNFα)和白细胞介素 6(IL 6)的含量。结果 :药物组TNFα和IL 6分别为 (0 .49± 0 .3 2 )、(0 .3 7±0 .15 )ng·mL 1,明显低于对照组 ,与正常组无差异。结论 :中药算盘子可以显著降低UC大鼠TNFα和IL 6的水平 。

Objective:The aim is to study the effects of the extract from Glochidion Puberum (GP) on the production of TNFα and IL 6 in macrophage from mice with ulcerative colitis (UC) induced by. Methods:The animal model of UC was made by filling the 5% CH 3COOH solution to the colon of 10 mice, who were randomly allocated to the control and treatment groups. 5 normal mice were used as normal control. 10 ml of normal saline were fed to the control and the normal control mice, twice a day for a total of 3 weeks before sacrifice. The same amount of Glochidion Puberum extract was fed to the mice in treatment group for the same period of time. The ELISA method was employed to measure TNFα and IL 6. Results:The contents of TNFα and IL 6 were 0.49±0.32 and 0.37±0.15, respectively in the control group and significantly lower than those in the controls. However, no significant difference was found between the control and the normal control mice. Conclusion:GP can significantly lower the TNFα and IL 6 in mice with UC and might be effective for treating UC.