摘要
In the previous work, we found that DAD 2 receptors in the spinal cord could me diat e significant anti hyperalgesic effect. But the mechanism is not clear. In the present experiments, the intrathecal injection and fluorescent in situ hybridiza tion (FISH) wi th fluorescentimmunohistochemical (FIH) double staining were used to observe the possible relationship between the anti hyperalgesic effects mediated by D 2 r eceptors and central opioid system. First, the effect of i.t. 5 mM naloxone on the anti hyperalgesia of i.t. D 2 rec eptor agonist LY171555 was examined, the results showed that naloxone produced c omplete antagonism on the anti hyperalgesia mediated by D 2 receptors in the s pinal cord, and suggested that opioid receptors may be activated when the D 2 r eceptors in the spinal cord mediated anti hyperalgesia. FISH with FIH double staining was used to look for the direct relationship betwe en D 2 receptors and the opioid system in the spinal cord. In dorsal horn, we o bserved the colocalization of D 2 mRNA and ENK neurons. The results showed that D 2 receptor mRNA and ENK IR double labeled cells were significantly increas ed at 8 hr after i.t. carrageenan. From these results, we concluded that the anti hyperalgesic effect mediat ed by D 2 receptors in the spinal cord is related to the opioid system, D 2 r eceptors may produce a direct action on ENK interneurons.
In the previous work, we found that DAD 2 receptors in the spinal cord could me diat e significant anti hyperalgesic effect. But the mechanism is not clear. In the present experiments, the intrathecal injection and fluorescent in situ hybridiza tion (FISH) wi th fluorescentimmunohistochemical (FIH) double staining were used to observe the possible relationship between the anti hyperalgesic effects mediated by D 2 r eceptors and central opioid system. First, the effect of i.t. 5 mM naloxone on the anti hyperalgesia of i.t. D 2 rec eptor agonist LY171555 was examined, the results showed that naloxone produced c omplete antagonism on the anti hyperalgesia mediated by D 2 receptors in the s pinal cord, and suggested that opioid receptors may be activated when the D 2 r eceptors in the spinal cord mediated anti hyperalgesia. FISH with FIH double staining was used to look for the direct relationship betwe en D 2 receptors and the opioid system in the spinal cord. In dorsal horn, we o bserved the colocalization of D 2 mRNA and ENK neurons. The results showed that D 2 receptor mRNA and ENK IR double labeled cells were significantly increas ed at 8 hr after i.t. carrageenan. From these results, we concluded that the anti hyperalgesic effect mediat ed by D 2 receptors in the spinal cord is related to the opioid system, D 2 r eceptors may produce a direct action on ENK interneurons.
出处
《针刺研究》
CAS
CSCD
2001年第3期213-214,共2页
Acupuncture Research
