白细胞介素13通过下调核因子-κB活化抑制系膜细胞白细胞介素1β的表达

Inhibitory effect of interleukin-13 on LPS-induced expression of interleukin-1βin mesangial cells by down-regulated activation of nuclear factor-κB

目的 研究白细胞介素１３（ＩＬ－１３）对脂多糖（ＬＰＳ）引起的肾小球系膜细胞核因子－κＢ（ＮＦ－κＢ）活化及白细胞介素１β（ＩＬ－１β）表达的影响，以探讨其抗炎作用的机制。方法 以体外培养的大鼠肾小球系膜细胞为靶细胞，用凝胶电泳迁移率试验（ＥＭＳＡ）测定ＮＦ－κＢ活性；用酶联免疫吸附法（ＥＬＩＳＡ）及逆转录聚合酶链反应（ＲＴ－ＰＣＲ）观察系膜细胞 ＩＬ－１β蛋白及基因表达。结果 ＬＰＳ可诱导系膜细胞ＮＦ－κＢ活化及ＩＬ－１β表达，这作用能被ＮＦ－κＢ抑制剂吡咯二硫氢基甲醋酯（ＰＤＴＣ）抑制。ＩＬ－１３亦有此抑制作用。结论ＩＬ－１３对于系膜细胞炎症反应的抑制作用可通过抑制ＮＦ－κＢ活化而实现。

Objective To examine the effect of interleukin-13(IL-13) on expression of interleukin-1β-(IL-1β) and activation of nuclear factorκB(NF-κB) induced by LPS in mesangial cells and to explore the anti-inflammatory mechanism of IL-13. Methods The protein synthesis of IL-1β in mesangial cells was measured by ELISA. The expression of IL-1β mRNA in mesangial cells was examined by RT-PCR. The activation of NF-κB in mesangial cells was determined by electrophoresis mobility shift assay(EMSA) . Results LPS induced activation of NF-κB and the expression of IL-1β in both protein and mRNA levels in mesangial cells. NF-κB inhibitor PDTC as well as IL-13 could inhibit the above effect of LPS. Conclusion Inhibitory effect of IL-l3 on IL-1β expression induced by LPS is mediated by, at least in part, down-regulation of NF-κB activation.