实验性肺心病时肺内一氧化氮的变化及作用

Changes and Role of Endothelium-derived Nitric Oxide in Experimental Pulmonary Heart Disease

为探索肺心病时肺内NO的变化及作用 ,利用免疫组织化学和图像分析方法 ,测定肺心病模型大鼠肺心病形成过程中补充L 精氨酸后 ,肺动脉内皮性一氧化碳合酶 (eNOS)的分布和变化。结果 :实验性肺心病形成过程中 ,肺动脉内皮细胞eNOS的染色增强 ;支气管上皮细胞内eNOS的免疫染色降低 ;补充L 精氨酸后损伤减轻。提示 ,NO在大鼠肺心病形成过程中具有重要的保护作用 ,其生成量增加 ,但与野百合碱 (MCT)引起的破坏比较 ,仍相对不足。

To study the changes of endothelium derived nitric oxide(EDNO) in pulmonary heart disease, adult male Wistar rats were given a single injection of monocrotaline (MCT) (60 μg/g) to build the model of pulmonary heart disease, or L arginine 〔500 μg/(g·d)〕 was injected at the same time. Three weeks later, the changes of rat lung were observed under light and electric microscopy, and the changes of endothelial nitric oxide synthase (eNOS) in rat lung were measured by image analysis. Three weeks after MCT treatment, the expression of eNOS increased significantly in endothelia of lung vessels, on the contrarary, decreased evidently in bronchial epithelia as the experimental time passed by, and L arginine could reduce the injury of lung induced by MCT. It showed that NO may play an important protective role during the process of pulmonary heart disease formed, the production of NO is not enough to resist the pulmonary vascular injury.