新生小鼠胸腺的体外生长及酶表达

The growth and enzymes expression of the thymuses of newborn mice in vitro

目的 :观察器官培养条件下新生小鼠胸腺的生长状况。方法 :对新生BALB/c小鼠胸腺分别进行 1、3、5和 7d的体外培养 ,然后用常规组织学及酶组织化学方法对其进行染色和镜下观察。结果 :从培养 1d组开始 ,胸腺的生长状况便可分为两个区 :第 1区为除贴附培养滤纸一侧以外的皮质部分 ,第 2区为髓质及贴附培养滤纸一侧的皮质部分。第 1区内细胞排列紧密 ,细胞呈良好的生长状态 ,有较多酶阳性反应较强的细胞 ;第 2区内细胞排列较松散 ,部分细胞结构较差 ,基质细胞明显减少 ,酶阳性反应细胞较少。以上生长状况在培养 3d组、5d组和 7d组更加明显 ,且 3组之间无明显差别。结论 :本器官培养条件下的胸腺生长状况具有区域性差别。

Objective: To observe the state of growth of the newborn mice's thymuses which grew in condition of organ culture. Methods: The thymuses of newborn BALB/c mice were cultured for 1 day, 3 days, 5 days and 7 days respectively and followed by staining with methods of conventional histology and enzyme histochemistry and were observed with microscope. Results: Since 1 day culture group each of the thymuses appeared two regions which showed different state of growth. The first region means the cortex besides the part which nestled closely to the culturing filter, the second region means medulla and the cortex which nestled closely to the culturing filter. The cells in first region arranged densely and were in fine state of growth. Quite many of cells which were strong enzyme positive were showed in this region, too. Cells in the second region arranged loose and part of them were poor in structures. Stromal cells decreased obviously, and only a little of cells which were enzyme positive were showed. The state of growth as described above were showed more obviously in 3 day culturing group, 5 day culturing group and 7 day culturing group, and there were no evident difference among the three groups . Conclusion: The state of growth of the thymuses which grew in condition of organ culture showed regional difference.