同型半胱氨酸和肾上腺髓质素在大鼠肺组织和气道平滑肌的相互作用

Interactions between homocysteine and adrenomedullin in rat lung tissue and airway smooth muscle cells

目的 :探讨肾上腺髓质素 (adrenomedullin ,AdM )对同型半胱氨酸 (homocysteine ,HCY)诱导的气道平滑肌细胞增殖的影响和HCY对肺组织合成分泌AdM的影响。方法 :在培养的大鼠气道平滑肌细胞上 ,测定3 H -TdR参入 ;用放免法测定肺组织和孵育液中AdM含量。结果 :0 .5mmol·L 1HCY刺激气道平滑肌细胞 (airwaysmoothmusclecells ,ASMC)增殖 ,AdM (10 9,10 8,10 7mol·L 1)呈浓度依赖方式抑制HCY诱导的ASMC增殖 ;HCY(0 .1,0 .5 ,1.0mmol·L 1)以浓度依赖方式诱导肺组织合成和分泌AdM ,超氧化物歧化酶 (superoxidedismutase ,SOD)可抑制其作用。结论 :在肺HCY和AdM呈相互作用关系 ,HCY调节AdM的生成 ,而AdM影响HCY的生物学效应。

Objective:To study the effect of Adrenomedullin (AdM) on the Homocysteine(HCY) induced proliferation of airway smooth muscle cells and the effect of HCY on the AdM synthesis and secretion in lung tissue.Methods:The 3 H TdR incorporation was measured in cultured airway smooth muscle cells(ASMC),and the content of AdM in lung tissue and incubative fluid were measured by a radioimmunoassay. Results:HCY (0.5 mmol·L -1 ) induced the proliferation of ASMC.AdM(10 -9 ,10 -8 ,10 -7 mol·L -1 ) inhibited the HCY induced proliferation of airway smooth muscle cells in a concentration dependent manner . HCY induced the AdM synthesis and secretion in lung tissue in a concentration dependent manner,and this effect was inhibited by superoxide dismutase (SOD). Conclusion: HCY and AdM influence each other in the lung, HCY regulates the production of AdM,which,in turn, inhibits the biological effect of HCY.