肾小管上皮细胞模拟缺血缺氧时黏附力的改变

Change of adhesion of renal tubular epithelial cells under mimic renal ischemia and anoxia

目的 直接测定模拟缺血缺氧时肾小管上皮细胞－基底膜黏附力。方法 用细胞流变学新手段──微管吸吮技术，对培养肾小管上皮细胞在模拟单纯缺血、单纯缺氧和同时缺血缺氧时与人工基底膜之间的影附力迸行测定。结果 模拟缺血缺氧时肾小管上皮细胞与人工基底膜之间黏附力较正常时明显降低（Ｐ＜０．００１）。结论 微管吸吮技术是定量研究肾小管上皮细胞黏附力及其改变的有效手段。肾小管上皮细胞与基底膜直接黏附力下降验证了国内外传统研究对其影附力改变的间接推测。

Objective To measure the adhesive forces of renal tubular epithelial cells(TEC) to artificial basement membrane (Matrigel) directly under mimic renal ischemia and anoxia. Methods By a new method of cellular rheology-micropipette aspiration technique, the adhesive forces of cultured TEC to Matrigel were measured directly under mimic renal ischemia and anoxia (including single ischemia, single anoxia and simultaneous ischemia and anoxia) . Results The direct adhesive forces of TEC to Matrigel decreased under renal ischemia and anoxia than that under normal condition (P < 0. 001). Conclusions Micropipette aspiration technique is an effective method to determine the TEC adhesion quantitatively. The decrease of adhesive forces proves the indirect estimation of TEC adhesion changes by other traditional methods in the pathogenesis and development of renal function impairment following renal ischemia.