番茄红素对人前列腺癌细胞生长的影响

The Effect of Lycopene on Cell Growth of Human Prostate Carcinoma Cell

目的 观察番茄红素对体外培养的人前列腺癌DU -145和LNCaP细胞存活率、细胞周期和凋亡的影响。方法 用MTT检测番茄红素对DU 145和LNCaP细胞作用的时间效应和剂量效应 ;流式细胞仪观察番茄红素处理人前列腺癌细胞后细胞周期以及细胞凋亡的改变。结果 番茄红素对DU 145细胞的增殖有明显抑制作用 ,抑制率可达 78% ,并有剂量 效应关系。流式细胞仪分析显示 ,番茄红素可影响该细胞周期并引起细胞凋亡 ,凋亡率最高达 42 42 %。而对LNCaP细胞作用不明显。结论 番茄红素对人前列腺癌细胞DU 145具有直接抑制作用 ,其抑制机理是通过影响人前列腺癌细胞的生长周期和诱导其凋亡而实现。本研究同时也显示番茄红素对激素不依赖型细胞DU

Objective To investigate the influence of lycopene on cell growth of prostate carcinoma cell line and apoptosis.Methods Time and dose effects of lycopene were detected using MTT assay;The influences in the cell cycle and the apoptosis were obsersved through flow cytometry.Resluts Lycopene could inhibit the growth of DU 154 cell line with a dose effect The biggest rate of cell growth inhibition is up to 78% in those treated with lycopene for 4 days.Lycopene can influence the cell cycly of CU 145 and apoptosis.But it has not been found in LNCaP cell line.Conclusion Lycopen inhibits DU 145 cell line in vitrol,and the mechanism may be related to the influence in the cell cycle and induction of apoptosis.It has been demonstrated that androgen indepent cell line(DU 145)are more sensitive to Lycopene than androgen dependent cell line(LNCaP).