分光光度法测定水果中Vc含量的研究

A Research on Measuring Content of Vitamin C in Fruits by using Spectrophotometer

提出一个利用Vc分子中的烯二醇基的还原作用将Fe(Ⅲ )定量还原为Fe(Ⅱ )与邻二氮菲显色。在波长5 10nm处 ,其颜色的深浅在一定范围内与Vc含量成正比。

A new method that measures the content of Vitamin C in fruits is proposed in this article. This method is based on the reductive effect of enediol group and reduces Fe (III) quantitatively to Fe (II) and colorate it with orthophenanthroline. At 510mm of the wavelength, the depth of color, within limits, corresponds with the content of Vitamin C.