摘要
为观察高糖状态下人类肾小球系膜细胞 (GMC)增殖及纤维连接蛋白 (FN)合成情况 ,体外培养GMC ,以噻唑蓝掺入法(MTT)和细胞计数观察GMC增殖 ,用RT PCR及双抗夹心ELISA方法检测FN的基因及蛋白质水平变化。结果发现 ,高糖对GMC增殖有抑制作用 ,对FN的合成呈浓度及作用时间依赖性增高。
To observe the high glucose concentration on proliferation and fibronectin (FN) synthesis of glomerular mesangial cells (GMC),a culture of human GMC was established and the cell proliferation was assessed using MTT method and cell counting, and the changes in FN was analyzed with RT PCR and ELISA. It was found that high glucose concentration inhibited GMC proliferation and increased FN synthesis in dose and time dependent manner.The results suggest that hyperglycemia may have important role in the development of diabetic nephropathy.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第1期30-31,共2页
Medical Journal of Chinese People's Liberation Army
基金
国家重点基础研究发展规划 973项目资助课题 (编号G2 0 0 0 0 5 70 0 3)