高糖促进肾小球系膜细胞纤维连接蛋白合成的实验研究

EFFECTS OF HIGH GLUCOSE CONCENTRATION ON FIBRONECTIN SYNTHESIS OF CULTURED HUMAN GLOMERULAR MESANGIAL CELLS

为观察高糖状态下人类肾小球系膜细胞 (GMC)增殖及纤维连接蛋白 (FN)合成情况 ,体外培养GMC ,以噻唑蓝掺入法(MTT)和细胞计数观察GMC增殖 ,用RT PCR及双抗夹心ELISA方法检测FN的基因及蛋白质水平变化。结果发现 ,高糖对GMC增殖有抑制作用 ,对FN的合成呈浓度及作用时间依赖性增高。

To observe the high glucose concentration on proliferation and fibronectin (FN) synthesis of glomerular mesangial cells (GMC),a culture of human GMC was established and the cell proliferation was assessed using MTT method and cell counting, and the changes in FN was analyzed with RT PCR and ELISA. It was found that high glucose concentration inhibited GMC proliferation and increased FN synthesis in dose and time dependent manner.The results suggest that hyperglycemia may have important role in the development of diabetic nephropathy.