15例甲型肝炎病人抗HAV-IgM和抗HAV-IgG的动态观察

STUDIES ON THE DYNAMIC CHANGES OF SERUM ANTI-HAV IgM AND IgG LEVELS IN 15 CASES OF HEPATITIS A

本文报道15例甲型肝炎病人从发病后2周到1年期间抗 HAV-IgM 和抗 HAV-IgG 的动态变化.实验结果表明抗 HAV-IgM 的滴度,高峰时为10^(-5)～10^(-7)。儿童在发病后3个月左右抗 HAV-IgM 全部转阴。部分成人则转阴时间相对长些。抗 HAV-IgG 高峰时最高滴度有10^(-8),一般为10^(-5)～10^(-7),有的病人在发病后3个月左右出现高峰,以后逐渐下降,也有出现高峰后持续不下降者。有的病人发病后2周即出现10^(-7),一年中持续高滴度,也有半年左右即降至临界值者.这反映了不同病人对 HAV 应答的多样性。本实验采用 ELISA 法,所用组织培养物经高度纯化后得到的 HAAg,增加了实验的敏感性和可靠性。

Serum anti-HAV IgM and IgG levels were determined by ELISA,using highly puri- fied HAV antigen from tissue culture,in 15 cases of hepatitis A 2 weeks to 1 year after onset of the disease.The peak titer of serum anti-HAV IgM was 10^(-5)—10^(-7).In chil dren,seroconversion to negative anti-HAV IgM occurred around 3 months after onset of the disease.In part of adults,anti-HAV IgM remained positive for longer time.The high est titer of serum anti-HAV IgG was 10^(-8),but in general the peak level was 10^(-5)—10^(-6) In most of the patierts the peak appeared around 3 months after onset of the disease.The titer could remain unchanged or decrease gradually.In some cases,anti-HAV IgG level rea- ched maximum in 2 weeks after onset of the disease and kept at high titer for 1 year.Our observation showed that there were different antibody responses to HAV in different patients.