参蒲汤抗小鼠癫痫的药效学研究

Experimental Pharmacodynamic Study on the Anti-Convulsion Effect of Shenpu Decoction

目的 :观察参蒲汤抗小鼠惊厥的效应。方法 :采用 3种方法造成的急性惊厥模型 (戊四唑惊厥模型 ,士的宁惊厥模型 ,最大电休克惊厥模型 ) ,通过这些模型的半数致惊量 (CD50 )来评价参蒲汤的抗惊厥效应 ,分组并分别用生理盐水 (对照组 ) ,参蒲汤 9g/kg、6g/kg、4g/kg(参蒲汤大、中、小剂量组 )和硝基安定(西药组 )治疗。结果 :在戊四唑模型中各组 (按上述顺序 )的CD50 分别为 (63 3± 3 4 )mg/kg、(116 2± 3 4 )mg/kg、(10 5 6± 3 7)mg/kg、(74 0± 3 7)mg/kg和 (197 2± 3 3)mg/kg ;在士的宁惊厥模型中各组的CD50分别为 (0 71± 0 0 4 )mg/kg、(1 2 1± 0 0 4 )mg/kg、(1 19± 0 0 4 )mg/kg、(0 94± 0 0 4 )mg/kg和 (1 16±0 0 4 )mg/kg ;最大电休克惊厥模型中各组的CD50 分别为 (67 1± 2 6)V ,>140V ,(10 9 4± 3 5 )V、(84 4±3 1)V和 (12 8 4± 3 9)V。结论

Objective: To observe the anti convulsion effect of Shenpu Decoction (SPD). Methods: Experiments were conducted in three acute convulsion models (cardiazol seizure, strychnine convulsion and maximal electrical shock). Models were divided into the control group (A, treated by normal saline), the high, middle and low dose SPD groups (B, C and D, treated with SPD 9g/kg, 6 g/kg and 4 g/kg respectively), and the nitrazepam treated group (E). The anti convulsion effect of SPD was evaluated by 50% convulsion dose (CD 50 ) detected in each group. Results: In cardiazol convulsion model, the CD 50 detected in group A-E were 63 3±3 4mg/kg, 116 2±3 4mg/kg, 105 6±3 7mg/kg, 74 0±3 7mg/kg and 197 2±3 3mg/kg respectively, while they were 0 71±0 04mg/kg, 1 21±0 04mg/kg, 1 19±0 04mg/kg, 0 94±0 05mg/kg and 1 16±0 04 mg/kg respectively in the strychnine convulsion model, and 67 1±2 6V, >140V, 109 4±3 5V, 84 4±3 1V and 128 4±3 9V in the maximal electrical shock model respectively. Conclusion: SPD has a good anticonvulsion effect.