浓当归注射液对肺纤维化发生发展过程的影响

Study of Influence of 25% Angelica Injection to Bleomycin-induced Pulmonary Fibrosis

目的 :探讨 2 5 %浓当归注射液对博莱霉素 (BLM)所致肺纤维化发生发展过程的影响及机理。方法 :大鼠气管内注入BLM诱导肺纤维化 ,随机分 3组 ,分别给于生理盐水、2 5 %当归注射液、氢化可的松腹腔内注射 ,第 2 ,7,15 ,30d分 4批处死 ,测算肺系数 ,肺组织HE染色、Masson3色染色、纤维连接蛋白 (Fn)免疫组化 ,及图象分析仪定量和电镜观察 ,测血及肺匀浆丙二醛 (MDA)、羟脯氨酸 (Hyp)。结果 :当归组和激素组 7d及 30d时肺系数显著低于对照组 ,肺泡炎及肺纤维化显著减轻 ,2d时Fn增多不明显 ,肺匀浆MDA产生显著减少 ,30d时血及肺匀浆Hyp均显著降低。结论

Abstract: To study the role of 25% angelica injection to Bleomycin induced pulmonary fibrosis. Methods: Rats were induced pulmonary fibrosis by injecting bleomycin intrabrochial once time.Three groups are divided into in random, were differently injected Natrium chloride 0.9% or 25% angelica injection or corticosteroids intraperitoneal everyday, and killed at 2,7,15,30 days.Lung moduli were measured, lung tissues were observed by HE, Masson, Fn immunohistochemical test by microscope and IBAS image analysis and scanning electron microscope.Serum and tissue MDA and Hyp were determined. Results: Angelica and corticosteroids can decrease lung moduli at 7 and 30 days(P< 0.05 ),obviously reduced alveolitis and pulmonary fibrosis.Fn and tissue MDA were lower than control group at 2 days.Serum and tissue Hyp were significantly lower at 30 days. Conclusion: Angelica can reduce pulmonary fibrosis by Bleomycin induced.