用多聚酶链反应直接检测鼻咽癌组织中EB病毒DNA

DETECTION OF THE EPSTEIN-BARR VIRUS DNA IN NASOPHARYNGEAL CARCINOMA WITH A POLYMERASE CHAIN REACTION METHOD

鼻咽癌患者体内 EB(Epstein-Barr)病毒的检测,目前常用血清学的方法,特异性和敏感性较差,对 WHO-Ⅰ型者阳性检出率亦较低,近年来用核素标记的探针进行分子杂交,可提高检出率,但需用放射性核素和较多的新鲜癌组织。本文介绍用多聚酶链反应(PCR)直接检测石蜡包埋鼻咽癌组织中 ER病毒 DNA 的方法。

A polymerase chain reaction method was used for detecting the Epstein-Barr virus(EBV)DNA from the formalin-fixed,paraffin-embedded tissues of nasopharyngeal carcinoma(NPC).Twenty-five NPC tissues of different pathological types,including WHO-I type inwhich detection of EBV by serological test often failed and some paraffin-embedded tissuesstored longer than ten years,were examined.Our amplification results showed that all the25 cases were EBV DNA positive.Therefore,this technique makes possible retrospectivestudies on the relationship between EBV ahd NPC.The method can also be used to analyzethe other pathogen DNA and genomic DNA in formalin-fixed,paraffin-embedded tissues.