天花粉蛋白对人PBMC分泌细胞因子水平及离子通道的影响

The effect of Trichosanthin on cytokines production and ion channel of human PB-MC

目的：研究天花粉蛋白对正常人外周血单个核细胞（PBMC）分泌细胞因子水平及其对T淋巴细胞离子通道的影响，揭示其免疫调节作用机理。方法：用放射免疫分析法检测天花粉蛋白对PBMC在植物血凝素-M（PHA－M）刺激下分泌IL-2、IL-6水平的影响；用膜片钳技术检测天花粉蛋白对T淋巴细胞膜电压依赖性钾通道的影响。结果：天花粉蛋白促进正常人PBMC在PHA－M刺激下分泌IL-2和IL-6；增大T淋巴细胞膜电压依赖性钾通道的电流强度。结论：天花粉蛋白可通过增大T淋巴细胞膜上电压依赖性钾通道电流强度及促进PBMC分泌IL-2和IL-6，增强机体免疫功能。

Abstract Objective: To study the effect of Trichosanthin on cytokines production and ion channel of human PBMC, to demonstrate its immune regulatory mechanism.Methods: The levels of IL-2 and IL-6 produced by PBMC under stimulation of PHA-M were measured by radio-immunoassay after cultured with Trichosanthin. The current intensity of voltage-dependent potassium channel [K(V) channel] were recorded by patch-clamp technique after added Trichosanthin.Results: Both IL-2 and IL-6 production were enhanced by Trichosanthin, and the current in-tensity of K(v) channel were markedly increased from (210.2 ?53.6)PA to (2 223.0 ?310.7)PA.Conclusion: Trichosanthin can enhance the production of both IL-2 and IL-6 of human PBMC and increase the current intensity of K( v) channel on T lymphocyte membrane, which im-prove cell-mediated immunity and humoral immunity.