Graves病患者甲状腺组织内催乳素mRNA的表达

Study on the expression of prolactin mRNA in thyroid glands of patients with Graves'disease

目的：研究Graves病（GD）患者甲状腺内有无催乳素（PRL）这一重要免疫调节激素的基因表达，以探讨此自身免疫反应发生部位能否产生PRL样物质。方法：应用原位杂交技术分别检测了GD甲状腺组织和非自身免疫甲状腺病标本（为对照组）内以及从GD甲状腺内提取的单个核细胞和原代培养的相应甲状腺滤泡细胞（TFC）中 PRL mRNA的表达。结果：GD甲状腺内浸润的单个核细胞和浸润部位的血管内皮细胞中可见到表达PRLmRNA的阳性细胞，但TFC均为阴性。而在对照组甲状腺内无PRLmRNA表达。从GD甲状腺内提取的单个核细胞中（9．8±3．3）％的细胞表达PRLmRNA，但原代培养的相应TFC均为阴性。结论：GD甲状腺组织内有 PRL mRNA表达，提示此局部能够产生 PRL样物质，后者可能通过自分泌和旁分泌方式影响GD的发生、发展。

Abstract Objective:To investigate whether mRNA of prolactin(PRL) ,an important immunomodulatory hormone,is expressed in thyroidglands of patients with Graves'disease(GD) .Methods:The expression of PRL mRNA was examined by nonisotopic in situ hybridization in GDthyroid glands and the control ones( including multinodular goiters and samples of normal thyroid tissue adjacent to adenomas) as well as intra-thyroidal mononuclear cells isolated from GD thyroid glands and primary thyroid follicular cell(TFC) cultures derived from them.Results:PRL mRNA was expressed in GD thyroid glands, but not in the control ones. It was localized in infiltrating mononuclear cells and vascular endothelial cells adjacent to mononuclear cell infiltrates, but not in TFC .(9.8?.3)% (x ?s) of intrathyroidal mononuclear cells isolated from GD thy-roid were found containing PRL mRNA.It was absent in the primary TFC cultures. Conclusion:PRL mRNA is expressed in GD thyroid tissue. This indicates that PRL-like substance can be locally produced in GD thyroid tissue, which may play an important role in GD pathogenesis by autocrine and paracrine mechanisms.