鼻咽癌组织细胞死亡受体DR4和DR5 mRNA的表达

Expression of cellular death receptors for DR4 and DR5 mRNA in nasopharyngeal cancer tissues

目的 分析鼻咽癌 (NPC)组织细胞死亡受体 (deathreceptor ,DR)DR4和DR5mRNA的表达。 方法从NPC癌组织提取的细胞总RNA逆转录生成第一条cDNA链后 ,再用DNA多聚酶链式反应扩增。经电泳、溴乙锭染色后 ,分析DR4和DR5受体mRNA的表达。结果 2 0例NPC癌组织有 12例可检测到DR4mRNA的表达 ,表达阳性率为 6 0 %。而所有 2 0例NPC癌组织均可检测到DR5mRNA的表达。 8例非NPC鼻咽组织也都可检测到DR5mRNA的表达 ,但未能检测到DR4mRNA的表达。结论 DR4受体mRNA在NPC癌组织与非NPC鼻咽组织之间表达不同 ,而DR5受体mRNA在NPC癌组织与非NPC鼻咽组织之间的表达无差别 ,提示DR4和DR5死亡受体在NPC癌组织与非NPC鼻咽组织可能起着不同的作用。

Objective To assay the expression of cellular death receptors for DR4 and DR5 mRNA in nasopharyngeal carcinoma (NPC) tissues. Methods Cellular total RNA isolated from NPC tissues was reversely transcribed into the first strand cDNAs, and the products were amplificated by PCR. After electrophoresising and dying with EB, the expression of DR4 or DR5 mRNA was analyzed. Results Twelve out of 20 NPC tissues were able to detect DR4 mRNA expression with a positive rate of 60%. In contrast, the expression of DR5 mRNA was present in all 20 NPC tissues. None of the 8 non-NPC nasopharyngeal tissues detected the expression of DR4 mRNA, but all of them expressed DR5 mRNA. Conclusion Between NPC tissue and non-NPC nasopharyngeal tissue, the expression of DR4 receptor mRNA is different. No difference is found in the expression of DR5 receptor mRNA. It implies that the receptors of DR4 and DR5 may play different roles between NPC tissue and non-NPC nasopharyngeal tissue.