摘要
通过电泳移动变化分析 (EMSA)及免疫组织化学 (IHC)的方法分别检测了 2 Gy和 0 .0 75 GyX射线照射的 EL- 4细胞内转录因子 NF- κB的 DNA结合活性的时程变化和 p6 5亚基胞浆胞核分布情况及 NF- κB抑制因子 IκBα水平的时程变化。结果表明 ,2 Gy和 0 .0 75 Gy X射线照射均可诱导 NF- κBp5 0 / p5 0和 p5 0 / p6 5的 DNA结合活性增强 ,但两种二聚体在两种剂量照射后的结合活性比值不同。0 .0 75 Gy照射诱导 p5 0 / p6 5活性增强为主 ,2 Gy照射诱导 p5 0 / p5 0活性增强为主。2 Gy和 0 .0 75 Gy均诱导 p6 5核转位增多和 IκBα的先降解后表达增多 ,但程度不同。提示不同剂量照射引起的转录调节变化不同 ,因此引起不同的细胞效应。
Changes in time course of the DNA binding activity of NF κB as well as the subcellular localization of p65 subunit and expression of IκBα in EL 4 cells after irradiation with 2 Gy and 0 075 Gy X rays were examined using electrophoresis mobility shift assay (EMSA) and immunohistochemistry (IHC), respectively Results showed that the increases in DNA binding activity of NF κB p50/p50 and p50/p65 were induced by both 0 075 Gy and 2 Gy X rays However, the amplitude of the increase in activity of the two dimers was different after irradiation with the two doses of X rays After irradiation with 0 075 Gy, the increase in p50/p65 activity was higher than that of p50/p50 activity After irradiation with 2 Gy, the situation was reversed Irradiation with both 2 Gy and 0 075 Gy induced an increase in the rate of p65 nuclear translocation and the degradation of IκBα before the increase of its expression, but the degree of these changes after different dose irradiation was different These results suggest that the transcriptional regulation of NF kB changes with irradiation dose, resulted in the difference in responses of cells (
出处
《辐射防护》
CAS
CSCD
北大核心
2002年第1期20-25,共6页
Radiation Protection
基金
国家自然科学基金资助项目 (392 70 2 0 7)
