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表面等离子体共振(SPR)生物传感器技术的进展—BIACORE J的应用 被引量:3

Recent Developments in SPR Biosensor Technology: Application of BIACORE J
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摘要 SPR生物传感器技术正在持续发展着.瑞典BIACORE公司新近推出了用于生物分子间相互作用日常分析的新型号仪器,BIACORE J.应用抗体/蛋白质A及配位基/受体等体系进行了BIACORE J用于活性浓度和键合亲和力测定的考核.结果表明了仪器的高灵敏度和键合响应的重现性.该型号仪器易于操作,并可用于多种领域,SPR技术也因而成为被广泛采用的研究工具. SPR biosensor technology continues to evolve.The recently released platform from Biacore AB (Uppsala,Sweden),BIACORE J,is designed for the routine analysis of biomolecular interactions.Using an antibody/protein A and a ligand/receptor system,we demonstrate the utility of BIACORE J in determining active concentration and binding affinities.The results from these studies illustrate the high sensitivity of the instrument and its ability to generate reproducible binding responses.The BIACORE J is easy to operate and useful in diverse applications,making SPR technology widely accessible as a research tool.
出处 《化学传感器》 CAS 2001年第2期14-23,共10页 Chemical Sensors
关键词 表面等离子体共振 SPR 生物分子 相互作用分析 生物传感器 BIACORE技术 surface plasmon resonance SPR biosensors biomolecular interaction analysis BIACORE technology
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参考文献1

  • 1Berit Johne. Epitope mapping by surface plasmon resonance in the BIAcore[J] 1998,Molecular Biotechnology(1):65~71

同被引文献142

  • 1于萍,崔小强,杨帆,黎拒难,杨秀荣.表面等离子体共振技术研究生物膜的进展[J].分析化学,2005,33(4):575-579. 被引量:5
  • 2Stabler G, Somekh M G, See C W. High- resolution wide-field surface plasmon microscopy [J]. J. Microsc., 2004, 214:328-333.
  • 3Matsubara K, Kawata S, Minami S. A compact surface -plasmon resonance sensor for measurement of water in process [J]. Appl. Spectrosc., 1988, 42:1375-1379.
  • 4Lyon L A, Holliway W D, Natan M J. An improved surface plasmon resonance imaging apparatus [J]. Rev.Sci. Instrum., 1999, 70:2076-2081.
  • 5Fu E, Chinowsky T, Foley J, et al. Rev. Sci. Instrum.,2004, 75:2300-2304.
  • 6Herminghaus S, Bechinger C, Petersen W, et al. Phase contrast surface mode resonance microscopy [ J ]. P Opt.Commun., 1994, 112:16-20.
  • 7O' Brien M J, Perez - Luna V H, Brueck S R J, et al. A surface plasmon resonance array biosensor based on spectroscopic imaging [J]. Biosens. Bioelectron, 2001,16:97 - 108.
  • 8www. ibis - spr. nl
  • 9www. gwcinstruments.com
  • 10Rothenhausler B, Knoll W. surface-plasmon microscopy [J]. Nature, 1988, 332:615 -617.

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