细胞吸附浓集法分离外环境水中脊髓灰质炎病毒

Isolation of Polioviruses in Environmental water with the Methed of Cell Absorption

〔目的〕建立一种用于检测外环境水中脊髓灰质炎病毒的方法。〔方法〕细胞吸附浓集法 :将自然沉清的水样抗菌、等渗处理后 ,加入适量的L2 0B细胞 ,混匀 ,充分吸收水中的脊髓灰质炎病毒 ,然后收集细胞培养、分离病毒。同时用AlCl3 -NaCl沉淀法浓集病毒 ,接种L2 0B细胞分离病毒 ,作为对照。〔结果〕40份水样 ,用L2 0B细胞吸附法检出 10株脊髓灰质炎病毒 ,用AlCl3 -NaCl沉淀法未检出脊髓灰质炎病毒。〔结论〕新建立的细胞吸附浓集法可用于外环境水中的脊髓灰质炎病毒的检测 ,且检测的敏感性明显高于AlCl3 -NaCl沉淀法。

Objective〕 To set up a simple, practical method for detecting the polio viruses in the environmental water.〔Methods〕 Adding L20B cells into the large volume water, which wa s sterilized with germicide and was balanced with balancing salts. Collecting th e cells after they fully reacted with polio viruses then culturing viruses, wit h the method of AlCl_3-NaCl sedimentation as control. 〔Results〕 O f 40 water samples, the polio viruses were detected in 10 with L20B cell absorption method, and no polio viruses were detected in all samples with the sedimentation method. 〔 Conclusions〕 The L20B cell absorption method might be used for detecing t he polio viruses in environmental water and it is more sensitive than the meth od of AlCl_3-NaCl sedimentation.