摘要
目的 :探讨川芎嗪对放射损伤小鼠骨髓基质细胞焦点黏附激酶 (FAK)表达的影响及其意义。方法 :利用流式细胞仪检测骨髓基质细胞的细胞周期及凋亡率 ;蛋白质免疫印迹 (Western blot)法检测骨髓基质细胞中 FAK的表达水平。结果 :放射损伤后骨髓基质细胞明显阻滞于 G0 G1期 ,凋亡率增加 ,同时 FAK的表达明显下降。用川芎嗪注射液治疗后骨髓基质细胞 S期合成活跃 ,凋亡率下降 ,FAK的表达增加。结论 :川芎嗪改善放射损伤小鼠骨髓微环境 ,促进骨髓基质细胞增生可能是通过增强
Objective:To evaluate the effect of ligustrazine(川芎嗪) on the expression of focal adhesions kinase(FAK) on bone marrow stromal cells in irradiation injured mice.Methods:The cell cycle and apoptosis of bone marrow stromal cells(BMSCs) were measured by flow cytometer;the expression of FAK on BMSCs was assayed by Western blot.Results:BMSCs were arrested in G0G1,percentages of apoptosis increased significantly after irradiation and the expression of FAK decreased.The recovery of bone marrow microenvironment was promoted by ligustrazine injection.Conclusions:The ligustrazine promoted the recovery of bone marrow microenvironment probably by increasing the expression of FAK on BMSCs.
出处
《中国中西医结合急救杂志》
CAS
2001年第5期266-267,共2页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
国家自然科学基金资助项目 (No.3 9870 92 6)
关键词
川芎嗪
放射损伤
焦点黏附激酶
骨髓微环境
ligustrazine
irradiation injury
focal adhesions kinase
bone marrow microenvironment
