补阳还五汤和有效部位组方对沙土鼠脑缺血及再灌注后脑组织EAA的影响

Effects of Bu Yang Huan Wu Decoction and its Active Fraction on Excitatory Amino Acid of Brain Tissue After Cerebral Ischemia and Reperfusion in Gerbils

目的 :比较研究补阳还五汤和其有效部位组方抗脑缺血及其再灌注损伤与脑组织兴奋性氨基酸 (EAA)的关系。方法 :采用沙土鼠双侧颈总动脉夹闭脑缺血模型 ,于缺血 15min和再灌注 48h取脑组织测定Glu、Asp、GABA和Gly。结果 :①缺血15min后 ,脑组织Glu和Asp含量明显升高 ,GABA含量升高 ,补阳还五汤可使脑组织Glu含量降低 ,有效部位组方可使脑组织Glu和Asp含量均降低 (P <0 0 5 )。②缺血 15min再灌注 48h后 ,脑组织Glu和Asp含量均显著升高 (P <0 0 5 ) ,补阳还五汤可使脑组织Glu含量降低 ,而有效部位组方可使脑组织Glu和Asp含量均降低 (P <0 0 5 )。结论 :兴奋性氨基酸的兴奋毒性参与了脑缺血及其再灌注后的脑损伤 ,补阳还五汤和其有效部位组方抗缺血性脑损伤的作用机理可能与其对抗脑缺血及其再灌注后EAA的升高有关。

To compare the effects of Bu Yang Huan Wu Deciction (BYHWD) and its active fraction on brain tissue following cerebral ischemia and reperfusion,Ceredral ischemia model in gerbils was indcued by ligating bilateral common carotid artery.The contents of glutamate (Glu),aspirate (Asp) and γ\|amino butyric acid,glycine in brain tissue were determined after cerebral ischemia for 15min followed by reperfusion for 48 hours.Results showed the contents of Glu,Asp and γ\|aminobutyric acid in brain tissue were markedly enhanced after cerebral ischemia for 15min.BYHWD decreased the contents of Glu in brain tissue remarkably;its active fraction reduced the contents of both Glu and Asp in brain tissue remarkably.The contents of Glu and Asp in brain tissue were remarkably increased after cerebral ischemia for 15min followed by reperfusion for 48 hours,BYHWD decreased the contents of Glu in brain tissue significantly,its active fraction reduced the contents of both Glu and Asp in brain tissue remarkably.