经动脉途径输注造血细胞提高同基因骨髓移植种植效率的实验研究

A Experimental Study on Improvement of the Seeding Efficiencies of Infused Donor Hematopoietic Cells in Syngeniec Bone Marrow Transplantation by Aortic Infusion

为了探讨提高骨髓移植种植效率的新方法 ,借助大鼠同基因骨髓移植模型 ,从动、静脉不同途径输注PKH 2 6荧光染料标记的供鼠骨髓细胞至受鼠体内 ,输注量为有核细胞数 2× 10 7 鼠。于骨髓移植后定时活杀受鼠 ,用流式细胞术检测归巢受鼠骨髓样本中PKH 2 6标记细胞。结果表明 ,于骨髓移植后 30小时动脉输注组和静脉输注组骨髓的造血细胞归巢指数分别为 14.5 2± 1.0 7和 10 .4 9± 0 .72。本研究证明 ,经动脉途径输注供鼠骨髓细胞比静脉途径输注将有更多的造血细胞在受鼠造血器官寄居。

In order to explore the improvement of seeding efficiencies of infused donor hematopoietic cells to bone marrow in bone marrow transplantation, two recipient groups of syngeneic rat model which received transplanted cells labeled with PKH-26, a red fluorescent membrane dye, by aortic or intravenous administration (2×10 7 nucleate cells per recipient rat)respectively, were assayed; at selected times following BMT, partial recipient rat were euthanized and then measured the numbers of PKH-26 labeled cells in recipient rat marrow samples by means of flow cytometry. The results showed that the homing indices of donor hematopoietic cells in aortic group and intravenous group were (14.52±1.07)% and (10.49±0.72)% at 30 hours after BMT, respectively (P<0.05). The results indicated that the number of donor hematopoietic cells localized to recipient bone marrow infused by aortic route is more than that infused by intravenous route.