P^(16)和PCNA在食管癌中的表达及意义

The expression and significance of P^(16) and PCNA in esophageal cancer.

陕西省肿瘤医院 ,西安 71 0 0 612 陕西省人民医院 ,西安 71 0 0 68目的 观察抑癌基因P16、增殖细胞核抗原PCNA在食管鳞状细胞癌中的表达特性及与临床病理联系。方法 应用SP免疫组织化学方法。结果 在 6 4例食管鳞癌中P16阳性表达率为 6 8 9% ,PCNA阳性细胞增殖指数 (PCNA PI)平均值 5 3 4。P16在低分化癌中较高分化癌阳性表达率低 ,相反 ,PCNA PI在高分化癌平均值低 ,在低分化癌高 (P <0 0 5 )。有淋巴结转移的P16阳性表达率低 ,且随着病期的进展阳性表达率降低 (P <0 0 5 )。P16阴性组PCNA PI高 (P >0 0 5 )。结论 联合检测P16蛋白和PCNA蛋白对食管鳞癌恶性度评定 ,治疗的选择 。

Objective To investigate the expression of anti oncogene P 16 and proliferative cell nuclear antigen (PCNA) in esophageal squamous cell cancer and their clinical pathology.Methods The SP immunohistochemistry method was used in the study.Results The positive P 16 expression was found in the 68.9% of the total 64 cases of esophageal squamous cell cancer, the average PCNA positive cell proliferation incidence(PCNA PI) was 53.4%. P 16 expression was very low in the poorly differentiated esophageal cancer. In contrast, the average PCNA PI was low in highly differentiated cancer cells, while being high in the poorly differentiated cells (P<0 05). Low P 16 expression was found while there was lymph node metastasis and as the cancer developed, the percentage of P 16 positive cell decreased (P<0 01). PCNA PI was low in the P 16 positive group and was high in the P 16 negative group (P>0 05).Conclusion Combined examination of the P 16 and PCNA is of great significance in determining the malignancy state, therapy method and evaluating the prognosis of the esophageal squamous cell cancer.