二氧化硫吸入对小鼠脑组织的氧化损伤

Oxidative Damage Induced by Sulfur Dioxide Inhalation in Brain of Mice

对昆明小鼠进行不同浓度SO2 吸入试验 ,然后测定脑组织中还原型谷胱甘肽 (GSH)含量、谷胱甘肽过氧化物酶 (GSH Px)活性、超氧化物歧化酶 (SOD)活性及脂质过氧化产物丙二醛 (MDA)含量 ,研究SO2 对小鼠中枢神经系统的氧化损伤作用 .雄鼠脑组织匀浆上清液GSH含量在SO2 浓度为 14mg m3 时明显上升 (P <0 0 5 ) ,浓度为 2 8、5 6和 84mg m3 时极显著降低 (P <0 0 1) ,而雌鼠在14和 2 8mg m3 时无明显变化 (P >0 0 5 ) ,在 5 6和 84mg m3 时极显著降低 (P <0 0 1) .雌雄小鼠的GSH Px活性在 14和 2 8mg m3 时无明显变化 (P >0 0 5 ) ,在 5 6、84mg m3 时均极显著降低 (P <0 0 1,P <0 0 0 1) .在SO2 上述 4种吸入浓度下 ,雌雄小鼠的SOD活性均显著降低 (P <0 0 5 )或极显著降低 (P <0 0 1,P <0 0 0 1) ;雄鼠和雌鼠的脂质过氧化产物丙二醛 (MDA)含量均极显著增高 (P <0 0 1,P <0 0 0 1) .结果表明 ,小鼠脑组织对SO2 的氧化损伤作用非常敏感 ,是SO2 的靶器官之一 ,SO2

Oxidative damage induced by SO 2 to brain of mice was examined.The various doses of SO 2 inhalation were 14 mg/m\+3,28 mg/m\+3,56 mg/m\+3 and 84 mg/m\+3. Experiments data showed to male mice, the content of GSH increased as a response to a stress of lower SO 2 doses, but decreased at higher doses; to female mice, the content of GSH did not change at lower SO 2doses and decreased at highter doses.The activity of GSH\|Px and SOD also decreased as a response on exposure to SO 2 inhalation. MDA increased sighificantly in various doses of SO 2 inhalation.These results domonstrated that the lipid peroxidation might play an important role in SO 2 toxicity to brain of mice.