摘要
将丙肝病毒C +E1区基因插入绿色荧光报告基因 pEGFP N1中 ,构建真核表达重组质粒 pEGFP N1 HCV/C +E1。转染小鼠骨髓瘤细胞SP2 / 0 ,在荧光显微镜下观察绿色荧光融合蛋白的表达情况 ,结果在细胞浆中出现了绿色荧光 ,表明目的基因得到表达。再通过G418筛选后大量培养用作细胞毒实验的靶细胞 。
A HCV/C+E 1 DNA fragment encoding core and envelope(E 1)proteins was inserted into a green fluorescent protein fusion vector to construct an eukaryotic expression recombinant plasmid,which has been transfected into mouse myeloma cell line SP2/0 by FuGENE TM 6 transfection reagent in vitro,and the green fluorescent emited by UV in SP2/0 cells was observed.It indicated the successful expression of aim genes.After screening with G 418 ,the transfected SP2/0 cells were cultured in a large amount as the target cell in detection of CTL activity.The result of cytotoxicity test suggested that EGFP could be made as a screening marker to apply to the preparation of target cell in detection of CTL activity.
出处
《微生物学免疫学进展》
2002年第1期42-43,共2页
Progress In Microbiology and Immunology
