EGR-1启动子在肿瘤基因治疗中的应用

Application of the early growth response-1 promoter in the research of tumor gene therapy

ＥＧＲ－１的启动子为早期生长反应因子－１基因上游约－５５０～０ｂｐ的一顺式作用元件，其活性受控于一些诱导剂，如电离辐射。联合ＥＧＲ－１的启动子与治疗基因（如ＴＮＦ－ａ、自杀基因），用辐射能在肿瘤局部从时、空调控治疗基因的表达，使其产物局限于肿瘤局部，并发挥放疗的杀肿瘤效应，而放疗与转基因产物又有协同作用。放射治疗与基因治疗的配伍为肿瘤的治疗提供了新思路。

EGR-1 promoter,a radiation-inducible promoter, is the cis-element(-550-0bp) of the early growth response-1 gene,its activity is regulated by inducers.such as ionizing radiation. The EGR-1 promoter/therapeutic gene(TNF-a,suicide gene) after irradiating can provide spatial and temporal control of the transgene expression and concentrate the transgene product to the tumor site. Radiation,the inducer of the EGR-1 promoter, is itself a therapeutic agent against cancer.There are synergistic effects between radiation and transgene product.Radiotherapy /gene therapy combination is one of the most promising approach in tumor gene therapy.