摘要
目的 :研究我国蚊虫中昆虫共生微生物Wolbachia的感染情况。方法 :采用依据Wolbachia的wsp基因序列建立的PCR分组检测方法 ,对我国蚊科中库蚊属、伊蚊属和按蚊属中一些蚊虫种类进行了检测。结果 :库蚊属中尖音库蚊复合组 4个亚种的实验室种群和伊蚊属中的白纹伊蚊的 4个地理株种群均有感染 ;感染的Wolbachia株属B组中的 pip组 ;同时在白纹伊蚊种群中还发现了有A组和B组Wolbachia的双重感染 ;而同时检测的三带喙库蚊、刺扰伊蚊、埃及伊蚊的 2个地理株、中华按蚊和斯氏按蚊没有发现Wolbachia的感染。结论 :本研究应用该方法 ,成功地对我国蚊虫体内感染的Wolbachia株进行了检测与分组。
Objective: To study the infection of Wolbachia in mosquitoes in China. Method: Specific primers derived from the wsp gene sequence were used to amplify DNA of Wolbachia from mosquitoes in China by polymerase chain reaction (PCR).Results: The characteristic 600bp products were observed after amplification of extracts from 4 subspecies of Culex pipiens complex and 4 geographical strains of Aedes albopictus. However,the products haven't been found in the strains of Aedes aegypti, Aedes vexans,Culex tritaeniorhynchus, Anopheles sinensis and Anopheles stephensi. The strains of Wolbachia which infect Culex pipiens complex and Ae. albopictus in China belong to pip group by means of the use of group-specific wsp PCR primers.It was found that there were double infections of A and B group Wolbachia in Ae.albopictus in China.Conclusion: The strains of Wolbachia infected in the mosquitoes in china have been successfully detected and classified by this method.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2002年第1期19-21,共3页
Chinese Journal of Vector Biology and Control
基金
国家自然科学基金资助项目 (No .39770 674)
