粘性放线菌Ⅱ型菌毛粘附与凝集活性的研究

A Study of Adherence and Aggregation properties of Type Ⅱ Fimbriae of Actinomyces Viscosus

目的:研究粘放菌Ⅱ型菌毛在细菌粘附与凝集过程中的作用。方法:制备粘放菌Ⅱ型菌毛多克隆抗体抗血清,检其对粘放菌变异株5519、5951、T14V的粘附抑制率,同时肉眼观察它对粘放菌与血链菌34间凝集反应的影响。结果:抗Ⅱ型毛多克隆抗体能减少5519、5951、T14V对玻壁的粘附量,并抑制5951、T14V与血链菌34间的凝集反应。结论:粘放菌Ⅱ型菌具有粘附与凝集的活性。

Objective:To study the effect of type Ⅱ fimbriae of Actinomyces viscosus on adherence and aggregation properties of the bacteria.Methods:Polyclonal antibodies against type Ⅱ fimbriae of Actinomyces viscosus were preparied in the study.The rate of inhibition adherence of 5519?5951?T14V were measured,and coaggregation between 5951?T14V and streptococcus sanguis 34 were observed.Results:The adherence ability of 5519?5951?T14V could be inhibited by the polyclonal antibodies,whereas the coaggregation between 5951?T14V and streptococcus sanguis 34 were also inhibited by the polyclonal antibodies.Conclusion:Type Ⅱ fimbriae of Actinomyces viscosus had adherence and aggregation properties.