重组犬钩虫分泌蛋白抗血清的免疫反应性

Studies on Immunological Reaction of the Antiserum of Recombinant Secreted Protein from Ancylostoma Caninum

目的 分析重组犬钩虫分泌蛋白抗血清与钩虫不同种、期抗原的免疫反应性。 方法 用微型垂直电泳槽进行 SDS- PAGE,以低分子量标准蛋白作参照。EL IB试验 :以重组犬钩虫分泌蛋白 - 1(Ac- r Asp- 1)或重组犬钩虫分泌蛋白 - 2 (Ac- r Asp- 2 )免疫鼠血清作第一抗体 ,羊抗鼠 Ig G- HRP作第二抗体 ,用 Western blotting发光底物试剂反应 ,全自动摄影 ,按照底片中显示带的位置测出相应分子量。 结果与结论 Ac- r Asp- 1组分为 45k Da,其免疫血清能识别犬钩虫第 期幼虫 (Ac- L3)抗原和 Ac- r Asp- 1,不与十二指肠钩虫成虫 (Ad- A)、十二指肠钩虫第 期幼虫 (Ad- L3)、美洲钩虫成虫 (Na- A)、犬钩虫成虫 (Ac- A)、巴西日圆线虫成虫 (Nb- A)抗原和 Ac- r Asp- 2起反应 ;Ac- r Asp- 2组分为 2 4k Da,其免疫血清能识别 Ad- A、Ad- L3、Na- A、Ac- A、Ac- L3抗原和 Ac- r Asp- 2 ,不与Nb- A抗原和 Ac- r Asp- 1起反应。

Objective To analyze the immunological reaction of the antiserum of recombinant secreted protein from Ancylostoma caninum with antigens of various species hookworms at different developmental stages. Methods SDS-PAGE and ELIB technique were employed in the study. Results and Conclusion The protein component of Ac-rAsp-1 was 45 kDa, its immune serum can recognize the antigens of Ac-L\-3 and Ac-rAsp-1 protein, but not react to the antigens of Ad-A?Ad-L\-3?Na-A?Ac-A?Nb-A and Ac-rAsp-2 protein. The protein component of Ac-rAsp-2 was 24 kDa, its immune serum can recognize the antigens of Ad-A?Ad-L\-3?Na-A?Ac-A?Ac-L\-3 and Ac-rAsp-2 protein, but not react to the antigens of Nb-A and Ac-rAsp-1 protein.