摘要
本文利用苯丙哌林结构中活性亚甲基与显色剂(对-二甲氨基苯甲醛)酸性溶液呈粉红色专属性反应,建立了苯丙哌林尿液浓度的可见光比色测定法。其最低检测浓度为5μg/ml,平均回收率为100.9±4.0(%),相关系数r=0.9994,回归方程y=0.0107x-0.0339,日内误差CV(%)=2.60,日间误差CV(%)=4.23.6名健康志愿者口服苯丙哌林片剂100mg,尿药测定结果的1g△x/△t-t曲线出现前高型双峰现象,即服药后7h出现第一高峰,此时平均尿药排泄速度为1.90±0.15μg/h,30h出现第二高峰,此时其平均尿药排泄速度为1.61±0.09μg/h.
A colorimetric method for the determination of Benproperine concentration in human urine is reported. P-dimethylaminobzal-dehyde was used as the colour reagent. The regression equation of Benproperine was: y=0.0107x-0.0339, r=0.9994. The mean recovery rate was 100.9±4.0(%). Precision studies for both within day and day to day at different concentrations provided CV(%) values of less than 6%. The detection limit of the method was 5μg per ml of urine. Two distinct absorption phases of Benproperine were noted in the urine of six healthy volunteers following an oral administration of 100mg, The Average values for the two peaks were 1.90±0.15μg/h and 1.61±0.09μg/h respectively.
关键词
苯丙哌林
比色法
尿药浓度
Benproperine
Colorimetric determination
Urine drug concentration
