乙型肝炎患者检测肝组织HBV-DNA的意义

The significance of detecting HBV DNA in liver tissue of HBV infectors

为了探讨提高乙型肝炎病毒微量检出率的有效途径。采用ＰＣＲ分别检测血清及肝穿刺组织ＨＢＶＤ－ＮＡ．血清检出总阳性率为 ４１．７％，肝组织检出总阳性率为 ７２．９％。在血清 ＨＢｓＡｇ阴性的病例中 ＰＣＲ血清检出阳性率为２６．７％，肝组织检出阳性率为６６．７％，由此可见ＰＣＲ检测灵敏、准确；特别对血清ＨＢＶＭ难以检测的微量ＨＢＶＤＮＡ更有价值。两种标本来源相比肝组织较血清为优。

To explore an effective passway to detect hepatitis B virus (HBV) genome. HBV DNA in serum and liver tissue were detected by PCR. The general positive rate of serum is 41 .7 , whereas, the general positive rate of liver tissue is 72.9%. In the HBsAg negative cases, the positive rate of serum is 26.7%, and that of liver tissue is 66.7%. The result suggested that PCR is a sensitive, specific method, which can be used to detect HBV DNA, and it is better to detect HBV DDN in liver tissue.