网纹甜瓜单倍体植株生产及倍数性鉴定(英文)

HAPLOID PLANT PRODUCTION AND PLOIDY LEVEL DETERMINATION IN NETTED MELON (Cucumis melo L.)

以软X 射线 (总辐射剂量 6 5 0Gy)照射过Cucumismelovar .momordica植株上的雄花花粉与网纹甜瓜杂种一代‘Rubia Red’(Cucumismelovar .reticulatusNaud .)已于上一日去雄的雌花授粉 ,经‘雌核发育’诱导形成单倍体胚。 3周后从采收的 4个幼果中获得种子 196 7粒 ,种子经灭菌后接种于 1/ 2MS +蔗糖30 g/L + gellangum 3g/L固体培养基上 ,3周后将萌发的 12个幼胚移植到MS +葡萄糖 2 0 g/L + gellangum3g/L培养基上培养。由假授精胚珠诱导成的 4株幼苗经倍数性分析仪 (PA型 ,德国Partec公司制造 )鉴定为单倍体植株。幼胚和单倍体植株的诱导率为 0 .6 %和 0 .2 %。通过茎节切段进行增殖培养 ,10周后获得 5

Gynogenetic haploid embryos in situ were induced through pollinating female flowers of hybrid F 1'Rubia Red' (Cucumis melo var. reticulatus Naud.) with soft X ray (650Gy) irradiated pollen from C. melo var. momordica plants. Twelve of 1967 seeds from four young fruits collected three weeks after pollination were germinated on the solid medium 1/2 MS+sucrose 30g/L+gellan gum 3g/L, four of which could grow into plantlets on medium MS+glucose 20g/L + gellan gum 3g/L. The four plantlets were identified to be haploids by use of ploidy analyser made in Germany. The inductivities of embryos and haploid plants were 0.6% and 0.2% respectively. Fifty four rooting regenerative plantlets were gained approximately ten weeks after propagation by cutting stem.