原发性高血压患者红细胞抗高血压因子对高血压大鼠血压的影响

EFFECTS OF ANTIHYPERTENSIVE FACTORFROM ERYTHROCYTES OF ESSENTIAL HYPERTENSIVE SUBJECTS ON BLOOD PRESSURE IN RATS

本工作观察了原发性高血压患者 (EHS) 红细胞抗高血压因子(AHF)对自发性高血压大鼠(SHR)和肾性高血压大鼠(RHR)及正常血压的 wistar Kyoto(WKY)大鼠和 wistar 大鼠的收缩压(SBP)和舒张压(DBP)的影响。结果如下:(1)从腹腔一次注入 AHF(1.6mg/kg体重),可明显降低 SHR 和 RHR 的 SBP。给 AHF10min 后,SHR 的 SBP 平均降低34.0 mmHg,至3h 恢复;RHR 在注射 AHF 后24h,SBP 平均降低92.5mmHg,且持续时间较长,至第九天仍维持在低水平。(2)从股静脉一次推注 AHF(0.8 mg/kg体重)后,SHR 和 RHR的 SBP 和 DBP 均有显著性降低,且对 RHR 作用时间较 SHR 长,对 DBP 作用时间较 SBP长。给 AHF 后12 min,SHR 的 SBP 和 DBP 分别降低42.8和48.2 mmHg;RHR 在给AHF 后25 min,SBP 和 DBP 分别降低38.3和42.5 mmHg;AHF 后5min,wistar大鼠 DBP 由96.7±12.9mmHg 降到 83.3±11.7 mmHg(P<0.05),而 SBP 无明显变化。AHF 的降压作用具有剂量依赖性。(3)AHF 可拮抗去甲肾上腺素对 Wistar 大鼠的升压作用。

The effects of antihypertensive factor(AHF)from erythrocytes of essentialhypertensive human subjects on the systolic blood pressure(SBP)and diastolic bloodpressure(DBP)in spontaneously hypertensive rats(SHR),renal hypertensive rats(RHR),Wistar-Kyoto rats(WKY)and Wistar rats were examined.Single intraperi-toneal injection of AHF(1.6 mg/kg body weight)resulted in a significant decreasein SBP of SHR and RHR.At 10 min postinjection,AHF lowered the SBP inSHR by 34.0 mmHg.SBP recovered to the original level at 3 h.The maximaldecrease of SBP in RHR by 92.5 mmHg was at 24h postadministration and theSBP did not recover until the 9th day.when AHF was administered via femoral vein(0.8 mg/kg body weight),themaximal decrease values of the SBP and the DBP were 42.8 and 48.2 mmHg inSHR at 12 min and 38.3 and 42.5mmHg in RHR at 25 min postinjection re-spectively.The DBP in Wistar rats decreased considerably(from 96.7±12.9 to83.3±11.7 mmHg)at 5 min postadministration of AHF,but no effect on DBPin WKY rats was observed.The depressor effect of AHF on SBP in RHR wasdose-dependent.AHF could also antagonize the pressor effect of norepinephrinein Wistar rats.