肾上腺皮质激素对心房钠尿肽基因表达的促进作用

EFFECT OF ADRENAL CORTICAL HORMONES ON THE EXPRESSION OF ATRIAL NATRIURETIC PEPTIDE(ANP) GENE IN VIVO

给完整的及切除肾上腺的雌性 Wistar 大鼠分別注射地塞米松、去氧皮质酮或地塞米松加去氧皮质酮;冷酚法提取心房总 RNA,用α-^(32P)标记的大鼠心房肽 cDNA 探针与之杂交。完整大鼠接受地塞米松和切除肾上腺后接受地塞米松加去氧皮质酮的大鼠,心房肽基因转录产物增加2倍,其余组无显著变化。结果提示糖皮质激素可促进心房肽基因表达,但此作用依赖于盐皮质激素的同时存在,单纯盐皮质激素不能增强该基因的表达。

Experiments were conducted in intact and adrenalectomized female Wistarrats given dexamethasone,deoxycorticosterone or both,and atria total RNA wasextracted with cold phenol method and hybridized to rat ANP-cDNA probe labeledwith α-~32P.The ANP gene transcripts in intact rats receiving dexamethasoneand in adrenalectomized rats receiving dexamethasone and deoxycorticosterone in-creased by 2-fold,while there were no significant changes in the other groups.Theresults suggest that glucocorticoids enhance ANP gene expression,an effect relyingon the presence of mineralocorticoids which given alone does not give rise to ANPgene expression enhancement.